How Many Reads You Need for RNA-Seq
A practical answer to RNA-seq depth: 30-40 million paired-end reads for whole-blood expression profiling, why the number moves with your question, and how to check that you got what you paid for.
Tag: sequencing-depth
2 posts
Read Depth in RNA-Seq: What It Means and How Much You Need
Read depth in RNA-seq is the number of reads assigned to your sample and, per gene, the number of reads supporting each transcript. This page explains how to compute it from your own files, why 30x means nothing here, and what depth to ask for.