Tag: nextflow

A step-by-step guide to turning raw FASTQ files from whole-genome and RNA sequencing into aligned, called, annotated, and validated results on your own hardware, with the specific tools, flags, and quality thresholds we use.

A practical pipeline for taking personal bulk RNA-seq FASTQs through quality control, quantification, normalization, and interpretation, with the parameters and failure modes that matter when n=1.

A 2026 guide to the RNA-seq workflow. Covers experimental design, data analysis and building reproducible pipelines.