How to Annotate Your Own RNA-Seq Data
A working guide to turning RNA-seq quantifications into an annotated, versioned gene table. Covers choosing an annotation, tx2gene and biotypes.
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A working guide to turning RNA-seq quantifications into an annotated, versioned gene table. Covers choosing an annotation, tx2gene and biotypes.
A practical guide to picking a human genome build and annotation for RNA-seq, building STAR and salmon indexes correctly, and checking that the reference you built is the one your quantification assumes.